year 11, Issue 1 (Spring 2023)
Ann Appl Sport Sci 2023, 11(1): 0-0 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Niazi S, Tayebi S M, Costa P B, Mirdar S, Hamidian G. Effects of Six Weeks of High-Intensity Interval Training on HIF-1α Protein Expression in Lung Tissues and Apoptosis of Pulmonary System in Male Wistar Rats. Ann Appl Sport Sci 2023; 11 (1)
URL: http://aassjournal.com/article-1-1203-en.html
URL: http://aassjournal.com/article-1-1203-en.html
1- Department of Exercise Physiology, Faculty of Physical Education and Sport Science, Kharazmi University, Tehran, Iran
2- Department of Exercise Physiology, Faculty of Sport Sciences, Allameh Tabataba'i University, Tehran, Iran , tayebism@atu.ac.ir
3- Department of Kinesiology, Human Performance Laboratory, Center for Sport Performance, California State University, Fullerton, CA, Fullerton, USA
4- Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Mazandaran University, Babolsar, Iran
5- Department of Basic Sciences, Division of Histology, University of Tabriz, Tabriz, Iran
2- Department of Exercise Physiology, Faculty of Sport Sciences, Allameh Tabataba'i University, Tehran, Iran , tayebism@atu.ac.ir
3- Department of Kinesiology, Human Performance Laboratory, Center for Sport Performance, California State University, Fullerton, CA, Fullerton, USA
4- Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Mazandaran University, Babolsar, Iran
5- Department of Basic Sciences, Division of Histology, University of Tabriz, Tabriz, Iran
Abstract: (927 Views)
Background. Apoptosis is a kind of regulated cell death involved in the normal growth, development, immune response, and elimination of abnormal cell proliferation in living organisms. In contrast, regular exercise protects against aging, lipid peroxidation, inflammation, and apoptosis.
Objectives. This study aimed to investigate the effects of 6 weeks of high-intensity interval training (HIIT) on HIF-1α levels in lung tissues and apoptosis of the pulmonary system in male Wistar rats.
Methods. Ten male Wistar rats were divided into training and control groups for this purpose. They performed six weeks of HIIT conducted in 5 sessions per week, each session for 30 minutes at a speed of 15 to 70 m/min. Then, animal lung tissue was extracted, and HIF-1α levels and lung tissue apoptosis index were measured via ELISA and immunohistochemical methods, respectively.
Results. showed that HIIT significantly increased HIF-1α level (p = 0.001) and lung and pulmonary duct apoptosis (p = 0.001) despite a significant improvement in exercise performance (p = 0.001).
Conclusion. According to the results, HIIT enhances athletic performance, HIF-1α level increases, and the rate of apoptosis rises.
Objectives. This study aimed to investigate the effects of 6 weeks of high-intensity interval training (HIIT) on HIF-1α levels in lung tissues and apoptosis of the pulmonary system in male Wistar rats.
Methods. Ten male Wistar rats were divided into training and control groups for this purpose. They performed six weeks of HIIT conducted in 5 sessions per week, each session for 30 minutes at a speed of 15 to 70 m/min. Then, animal lung tissue was extracted, and HIF-1α levels and lung tissue apoptosis index were measured via ELISA and immunohistochemical methods, respectively.
Results. showed that HIIT significantly increased HIF-1α level (p = 0.001) and lung and pulmonary duct apoptosis (p = 0.001) despite a significant improvement in exercise performance (p = 0.001).
Conclusion. According to the results, HIIT enhances athletic performance, HIF-1α level increases, and the rate of apoptosis rises.
Full-Text [PDF 475 kb]
(336 Downloads)
APPLICABLE REMARKS
APPLICABLE REMARKS
• High-intensity interval training (HIIT) can induce lung tissue but increases the performance in male Wistar rats.
Type of Study: Original Article |
Subject:
Sport Physiology and its related branches
Received: 2022/05/23 | Accepted: 2022/08/1
Received: 2022/05/23 | Accepted: 2022/08/1
References
1. Tayebi SM, Krüger K, Ebrahimi F, Izadi A, Roushan M, Nenasheva AV. Supplementation with Ziziphus Jujuba Suppresses Apoptosis Signals in Neutrophils after Acute Exercise. Montenegrin Journal of Sports Science and Medicine. 2021;10(2):Ahead of Print. [DOI:10.26773/mjssm.210905]
2. Solary E, Dubrez L, Eymin B. The role of apoptosis in the pathogenesis and treatment of diseases. European Respiratory Journal. 1996;9(6):1293-305. [DOI:10.1183/09031936.96.09061293] [PMID]
3. Piret J-P, Mottet D, Raes M, Michiels C. Is HIF-1α a pro-or an anti-apoptotic protein? Biochemical pharmacology. 2002;64(5-6):889-92. [DOI:10.1016/S0006-2952(02)01155-3] [PMID]
4. Pullamsetti SS, Mamazhakypov A, Weissmann N, Seeger W, Savai R. Hypoxia-inducible factor signaling in pulmonary hypertension. The Journal of Clinical Investigation. 2020;130(11). [DOI:10.1172/JCI137558] [PMID] [PMCID]
5. Scholz CC, Taylor CT. Targeting the HIF pathway in inflammation and immunity. Current opinion in pharmacology. 2013;13(4):646-53. [DOI:10.1016/j.coph.2013.04.009] [PMID]
6. Chimenti L, Morici G, Paternò A, Bonanno A, Siena L, Licciardi A, et al. Endurance training damages small airway epithelium in mice. American journal of respiratory and critical care medicine. 2007;175(5):442-9. [DOI:10.1164/rccm.200608-1086OC] [PMID]
7. Vieira RP, de Andrade VF, Duarte ACS, Dos Santos AB, Mauad T, Martins MA, et al. Aerobic conditioning and allergic pulmonary inflammation in mice. II. Effects on lung vascular and parenchymal inflammation and remodeling. American Journal of Physiology-Lung Cellular and Molecular Physiology. 2008;295(4):L670-L9. [DOI:10.1152/ajplung.00465.2007] [PMID]
8. Margaritis I, Palazzetti S, Rousseau A-S, Richard M-J, Favier A. Antioxidant supplementation and tapering exercise improve exercise-induced antioxidant response. Journal of the American College of Nutrition. 2003;22(2):147-56. [DOI:10.1080/07315724.2003.10719288] [PMID]
9. Pedersen BK, Steensberg A. Exercise and hypoxia: effects on leukocytes and interleukin-6-shared mechanisms? Medicine & Science in Sports & Exercise. 2002;34(12):2004-12. [DOI:10.1097/00005768-200212000-00022] [PMID]
10. Dayan F, Mazure NM, Brahimi-Horn MC, Pouysségur J. A dialogue between the hypoxia-inducible factor and the tumor microenvironment. Cancer Microenvironment. 2008;1(1):53-68. [DOI:10.1007/s12307-008-0006-3] [PMID] [PMCID]
11. Li K, Qiu H, Yan J, Shen X, Wei X, Duan M, et al. The involvement of TNF-α and TNF-β as proinflammatory cytokines in lymphocyte-mediated adaptive immunity of Nile tilapia by initiating apoptosis. Developmental & Comparative Immunology. 2021;115:103884. [DOI:10.1016/j.dci.2020.103884] [PMID]
12. Gleeson M, McDonald W, Cripps A, Pyne D, Clancy R, Fricker P. The effect on immunity of long‐term intensive training in elite swimmers. Clinical & Experimental Immunology. 1995;102(1):210-6. [DOI:10.1111/j.1365-2249.1995.tb06658.x] [PMID] [PMCID]
13. Zagórska A, Dulak J. HIF-1: the knowns and unknowns of hypoxia sensing. Acta Biochimica Polonica. 2004;51(3). [DOI:10.18388/abp.2004_3545] [PMID]
14. Semenza GL. Hydroxylation of HIF-1: oxygen sensing at the molecular level. Physiology. 2004;19(4):176-82. [DOI:10.1152/physiol.00001.2004] [PMID]
15. Truong L, Zheng Y-M, Kandhi S, Wang Y-X. Overview on Interactive Role of Inflammation, Reactive Oxygen Species, and Calcium Signaling in Asthma, COPD, and Pulmonary Hypertension. Lung Inflammation in Health and Disease, Volume II: Springer; 2021. p. 147-64. [DOI:10.1007/978-3-030-68748-9_9] [PMID]
16. Yue J, López JM. Understanding MAPK signaling pathways in apoptosis. International journal of molecular sciences. 2020;21(7):2346. [DOI:10.3390/ijms21072346] [PMID] [PMCID]
17. Weidemann A, Johnson R. Biology of HIF-1 α. Cell Death & Differentiation. 2008;15(4):621-7. [DOI:10.1038/cdd.2008.12] [PMID]
18. Garvey J, Taylor C, McNicholas W. Cardiovascular disease in obstructive sleep apnoea syndrome: the role of intermittent hypoxia and inflammation. European Respiratory Journal. 2009;33(5):1195-205. [DOI:10.1183/09031936.00111208] [PMID]
19. Saikumar P, Dong Z, Patel Y, Hall K, Hopfer U, Weinberg JM, et al. Role of hypoxia-induced Bax translocation and cytochrome c release in reoxygenation injury. Oncogene. 1998;17(26):3401-15. [DOI:10.1038/sj.onc.1202590] [PMID]
20. Milton SL. Conditioning Medicine.
21. Morishima N, Nakanishi K, Takenouchi H, Shibata T, Yasuhiko Y. An endoplasmic reticulum stress-specific caspase cascade in apoptosis: cytochrome c-independent activation of caspase-9 by caspase-12. Journal of Biological Chemistry. 2002;277(37):34287-94. [DOI:10.1074/jbc.M204973200] [PMID]
22. Kim J-Y, Park J-H. ROS-dependent caspase-9 activation in hypoxic cell death. FEBS letters. 2003;549(1-3):94-8. [DOI:10.1016/S0014-5793(03)00795-6] [PMID]
23. Chen D, Li M, Luo J, Gu W. Direct interactions between HIF-1α and Mdm2 modulate p53 function. Journal of Biological Chemistry. 2003;278(16):13595-8. [DOI:10.1074/jbc.C200694200] [PMID]
24. Dong Z, Wang JZ, Yu F, Venkatachalam MA. Apoptosis-resistance of hypoxic cells: multiple factors involved and a role for IAP-2. The American journal of pathology. 2003;163(2):663-71. [DOI:10.1016/S0002-9440(10)63693-0] [PMID]
25. An WG, Kanekal M, Simon MC, Maltepe E, Blagosklonny MV, Neckers LM. Stabilization of wild-type p53 by hypoxia-inducible factor 1α. Nature. 1998;392(6674):405-8. [DOI:10.1038/32925] [PMID]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
