en ورزش، تمرین و سلامتی Exercise, Training and Health مقاله اصیل Original Article <div style="text-align: justify;"><strong>Background. </strong>Persistent physical fatigue (PPhF) is one of the most common issues in athletes; salivary human herpes virus 6 and/or 7 (HHV-6/7) have recently attracted attention as novel microbiological markers for its assessment.<br> <strong>Objectives. </strong>We aimed to investigate potential variabilities of salivary HHV-6/7 levels in athletes, along with traditional assessments.<br> <strong>Methods. </strong>We conducted an observational study over two weeks including 54 healthy male university athletes (n = 44) and non-athletes (n = 10). We used a questionnaire on daily life to monitor the participants&rsquo; states and habits. The main measures were salivary HHV-6/7 and secretory immunoglobulin A (SIgA) levels, and total mood disturbance (TMD) scores in the Profile of Mood States.<br> <strong>Results. </strong>In the questionnaire on daily life, subjective fatigue increased in the athletes (p < 0.05), while the health condition and daily habits did not change in either group (p > 0.05); accordingly, fatigue may be due to training-induced physical stressors rather than pathological events or changes in daily habits. Salivary HHV-6 levels increased only in the athletes after one week (p < 0.05), whereas salivary HHV-7 and SIgA levels, and TMD scores remained unchanged (p > 0.05).<br> <strong>Conclusion. </strong>These findings suggested that salivary HHV-6 may be a more sensitive marker of PPhF than others.</div> Fatigue, Saliva, Biomarker, Infection, Immune Function 0 0 http://aassjournal.com/browse.php?a_code=A-11-1827-1&slc_lang=en&sid=1 Shinsuke Tamai tama1994@outlook.jp 100319475328460011399 100319475328460011399 No Doctoral Program in Sports Medicine, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Japan Ryota Sone r.sone0311@gmail.com 100319475328460011400 100319475328460011400 No Department of Sport Research, Japan Institute of Sports Sciences, Kita-ku, Japan Akari Kitahara akarikit111@gmail.com 100319475328460011401 100319475328460011401 No Doctoral Program in Sports Medicine, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Japan Kai Aoki fineday0126@gmail.com 100319475328460011402 100319475328460011402 No Laboratory of Laboratory/Sports Medicine, Division of Clinical Medicine, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan. Japan Society for the Promotion of Science, Chiyoda-ku, Japan Takehito Sugasawa take0716@krf.biglobe.ne.jp 100319475328460011403 100319475328460011403 No Laboratory of Laboratory/Sports Medicine, Division of Clinical Medicine, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan Kazuhiro Takekoshi k-takemd@md.tsukuba.ac.jp 100319475328460011404 100319475328460011404 No Laboratory of Laboratory/Sports Medicine, Division of Clinical Medicine, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan Koichi Watanabe watanabe.koichi.ga@u.tsukuba.ac.jp 100319475328460011405 100319475328460011405 Yes Faculty of Health and Sport Sciences, University of Tsukuba, Tsukuba, Japan