Annals of Applied Sport Science
تازههای علوم کاربردی ورزش
Ann Appl Sport Sci
Medical Sciences
http://aassjournal.com
1
admin
2476–4981
2322-4479
10.61186/aassjournal
en
jalali
1397
1
1
gregorian
2018
4
1
6
1
online
1
fulltext
en
Are Heart Muscle Irisin Levels and FNDC5 Gene Expression Regulated by Endurance and Resistance Exercises?
فيزيولوژی ورزشی و شاخههای وابسته بدان
Sport Physiology and its related branches
مقاله اصیل
Original Article
<div style="text-align: justify;"><span style="font-family:times new roman;"><strong>Background.</strong> Irisin is the result of the proteolysis of fibronectin type III domain-containing protein 5, the levels of which are determined by physical activity.<br>
<strong>Objective(s). </strong>This study aims to identify changes in FNDC5 gene expression and the heart muscle irisin levels in male rats following 8 weeks resistance and endurance training</span><span style="font-size:12px;"><span style="font-family:times new roman;">.<br>
<strong>Methods. </strong>Fifteen male rats (10-12 weeks old and 331.8±63.09 g in weight) were randomly assigned to three groups: control, resistance </span></span><span style="font-size:12px;"><span style="font-family:times new roman;">and</span></span><span style="font-size:12px;"><span style="font-family:times new roman;"> endurance training groups (5 in each group). The endurance group performed activities on a treadmill for 8 weeks. The resistance group performed activities for 8 weeks on a one-meter-long ladder with a slope of 85○. The control group did not undergo any particular training. Sampling was carried out 72 h after the end of the training. The heart tissue </span></span><span style="font-size:12px;"><span style="font-family:times new roman;">were</span></span><span style="font-size:12px;"><span style="font-family:times new roman;"> excised, cleaned and immediately frozen in liquid nitrogen, and stored at -70○C. FNDC5 gene expression and the heart muscle irisin levels were measured using Real-Time PCR and ELISA, respectively.<br>
<strong>Results. </strong>Kruskal-Wallis test showed a significant increase in the heart muscle FNDC5 expression in both training groups (P=0.004). One-way ANOVA showed a significant difference in heart muscle irisin levels among the groups (p=0.004).<br>
<strong>Conclusion. </strong>It can be concluded that both resistance and endurance training can significantly increase FNDC5 gene expression and the heart muscle irisin levels, suggesting that physical activity can prevent and cure cardiovascular diseases.</span></span></div>
Resistance Training, Endurance Training, FNDC5, Irisin
21
28
http://aassjournal.com/browse.php?a_code=A-11-545-3&slc_lang=en&sid=1
Mohammad
Hosseinzadeh
mhzadehsport@gmail.com
10031947532846007046
10031947532846007046
No
Department of Physical Education, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran
Amir
Rashid Lamir
rashidlamir@um.ac.ir
10031947532846007047
10031947532846007047
Yes
Department of Exercise Physiology, Faculty of Sport Science, Ferdowsi University of Mashhad, Mashhad, Iran
Seyed Mahmud
Hejazi
Sm.hejazi37@gmail.com
10031947532846007048
10031947532846007048
No
Department of Physical Education, Mashhad Branch, Islamic Azad University, Mashhad, Iran