year 6, Issue 3 (Autumn 2018)                   Ann Appl Sport Sci 2018, 6(3): 1-5 | Back to browse issues page

XML Print

1- Department of Molecular Biology and Genetics, Faculty of Engineering and Natural Sciences, Üsküdar University, Istanbul, Turkey
2- Department of Molecular Biology and Genetics, Faculty of Engineering and Natural Sciences, Üsküdar University, Istanbul, Turkey. Department of Medical Biology and Genetics, Faculty of Dentistry, Marmara University, Istanbul, Turkey ,
Abstract:   (6795 Views)
Background. The determination of the genetic endowment of athletic performance in sports is an important step in developing personal training sessions or nutritional supplements for success in sports. Information about the genetic parameters responsible for these metabolisms will help sport’s scientist to develop new insights for better performance. Muscle metabolism is one of the key points in better personal athletic performance.
Objectives. The aim of this study is to analyze the distribution of the methylenetetrahydrofolate reductase enzyme (MTHFR) rs1801133 (C677T) genotype and allele distribution in a Turkish professional cyclist cohort.
Methods. There were 25 Turkish cyclists enrolled in the study. Peripheral blood used for DNA isolation and the conventional polymerase chain
reaction- restriction fragment length polymorphism (PCR- RFLP) methodology were used for genotyping.
Results. There were 14 (56%), 10 (40%) and 1 (4%) cyclist who had CC, CT, and TT genotypes, respectively. C allele was counted as 38 (76%), and T alleles as 12 (24%). 9 (50%) of the male cyclist had CC, 8 (44.4%) had CT and only 1 had TT (5.6%) genotypes. C allele was counted as 26 (72.2%), and the T allele as 10 (27.8%)
in the male cyclists. In the females, the respective genotypes for CC and CT were 5 (71.4%) and 2 (28.6%). C allele was counted as 12 (85.7%) and T allele as 2 (14.3%).
Conclusion. In our cohort, both of the two genders, the CC genotype 
and C allele were found to be higher when compared to the other genotypes and T allele. Larger prospective studies focusing on the influence of MTHFR rs1801133 polymorphism in athletic performance are required for confirmation of our findings.
Full-Text [PDF 587 kb]   (1501 Downloads)    
• Determining the MTHFR C677T genotype will be helpful for trainers, coaches, and cyclists when setting up training and recovery time sessions. Also, having information about MTHFR C677T will be helpful for folic acid or multivitamin supplementation.

Type of Study: Brief Report | Subject: Sport Physiology and its related branches
Received: 2018/01/25 | Accepted: 2018/07/30

1. Ulucan K, Yalcin S, Akbas B, Uyumaz F, Konuk M. Analysis of Solute Carrier Family 6 Member 4 Gene promoter polymorphism in young Turkish basketball players. The journal of Neurobehavioral Sciences. 2014;1(2):37-40. [DOI:10.5455/JNBS.1403730925]
2. Ulucan K. Literature Review of Turkish Sportsmen in Terms of ACTN3 R577X Polymorphism. Clinical and Experimental Health Sciences. 2016;6(1):44-7. [DOI:10.5152/clinexphealthsci.2016.059]
3. Lanferdini FJ, Bin RF, Cunha GS, Lopes AL, Castro FAS, Oliveira AR, et al. Relationship between physiological and biomechanical variables with aerobic power output in Cycling. Journal of Science and Cycling. 2014;3(1):2-8.
4. Ulucan K, Karahan M, Sağlam E. Biochemical and molecular effects of folic acid metabolism to Parkinson, Alzheimer, bipolar and schizophrenic disorders. Anatolian Journal of Psychiatry. 2013;14(4):378- 82. [DOI:10.5455/apd.40636]
5. Goyette P, Sumner JS, Milos R, Duncan AM, Rosenblatt DS, Matthews RG, et al. Human methylenetetrahydrofolate reductase: isolation of cDNA, mapping and mutation identification. Nature Genetics. 1994;7(2):195-200. [DOI:10.1038/ng0694-195] [PMID]
6. Uğuz N, Erden G, Güngör O, Bal C, Yıldırımkaya M. Determination of the frequency of MTHFR C677T and MTHFR A1298C polymorphisms in persons with polymorphic MTHFR gene. Journal of Clinical and Experimental Investigations. 2012;3(4):472-6. [DOI:10.5799/ahinjs.01.2012.04.0205]
7. Terruzzi I, Senesi P, Montesano A, La Torre A, Alberti G, Benedini S, et al. Genetic polymorphisms of the enzymes involved in DNA methylation and synthesis in elite athletes. Physiological genomics. 2011;43(16):965-73. [DOI:10.1152/physiolgenomics.00040.2010] [PMID]
8. Barres R, Zierath JR. DNA methylation in metabolic disorders. The American journal of clinical nutrition. 2011;93(4):897s-900. [DOI:10.3945/ajcn.110.001933] [PMID]
9. Dinc N, Yucel SB, Taneli F, Sayin MV. The effect of the MTHFR C677T mutation on athletic performance and the homocysteine level of soccer players and sedentary individuals. Journal of human kinetics. 2016;51:61-9. [DOI:10.1515/hukin-2015-0171] [PMID] [PMCID]
10. Banting LK, Pushkarev VP, Cieszczyk P, Zarebska A, Maciejewska-Karlowska A, Sawczuk MA, et al. Elite athletes' genetic predisposition for altered risk of complex metabolic traits. BMC genomics. 2015;16:25. [DOI:10.1186/s12864-014-1199-0] [PMID] [PMCID]
11. Castro R, Rivera I, Ravasco P, Jakobs C, Blom HJ, Camilo ME, et al. 5,10-Methylenetetrahydrofolate reductase 677C-->T and 1298A-->C mutations are genetic determinants of elevated homocysteine. QJM : monthly journal of the Association of Physicians. 2003;96(4):297-303. [DOI:10.1093/qjmed/hcg039] [PMID]
12. Zarebska A, Ahmetov, II, Sawczyn S, Weiner AS, Kaczmarczyk M, Ficek K, et al. Association of the MTHFR 1298A>C (rs1801131) polymorphism with speed and strength sports in Russian and Polish athletes. Journal of sports sciences. 2014;32(4):375-82. [DOI:10.1080/02640414.2013.825731] [PMID]
13. Barres R, Yan J, Egan B, Treebak JT, Rasmussen M, Fritz T, et al. Acute exercise remodels promoter methylation in human skeletal muscle. Cell metabolism. 2012;15(3):405-11. [DOI:10.1016/j.cmet.2012.01.001] [PMID]
14. Coffey VG, Hawley JA. The molecular bases of training adaptation. Sports medicine (Auckland, NZ). 2007;37(9):737-63. [DOI:10.2165/00007256-200737090-00001]

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.