year 6, Issue 2 (Summer 2018)                   Ann Appl Sport Sci 2018, 6(2): 37-44 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Rahmaty S, Gaeini A A, Dolatshahi M, Choobineh S. The Effect of High-Intensity Interval Training and Continuous Training on the Desnutrin Gene Expression in the Subcutaneous Adipose Tissue and the Quadriceps Femoris Muscle Tissue of Obese Male Rats. Ann Appl Sport Sci 2018; 6 (2) :37-44
1- Department of Exercise Physiology, Faculty of Sport Sciences, University of Tehran, Tehran, Iran ,
2- Department of Exercise Physiology, Faculty of Sport Sciences, University of Tehran, Tehran, Iran
3- Department of Medical Physiology, Faculty of Medical Sciences, Dezful University of Medical Sciences, Dezfoul, Iran
Abstract:   (5593 Views)
Background. Desnutrin is an enzyme that catalyzes the first step of cytoplasmic triacylglycerol lipolysis from white adipose tissue and several other tissues, which are disrupted by the obesity and metabolic syndrome.
Objectives. The aim of this study was to compare the effect of high-intensity interval training and continuous training on the
desnutrin gene expression in the subcutaneous adipose tissue and the quadriceps muscle tissue of obese male rats.
Methods. For this purpose, after 12 weeks
on a high-fat diet, 30 Wistar rats were divided into two groups: continuous training (n =10) and high-intensity interval training (n=10). Continuous (80%VO2max) and high-intensity interval exercise training (100%VO2max) sessions were conducted according to the principle of overload for six weeks and six sessions per week. Forty-eight hours after the last training session, the abdominal subcutaneous adipose tissue and quadriceps muscle tissue were extracted, and the expression level was assessed using the RT-PCR method.
Results. The study showed that the difference between high-intensity interval and continuous training on the
desnutrin gene expression subcutaneous adipose tissue was significant (p=0.004), but there were no significant differences (p=0.415) between them in the quadriceps femoris muscle tissue.
Conclusion. According to the findings of the present study
, in
the adipose tissue, continuous training has a greater effect on the desnutrin gene expression than high-intensity interval training. In the muscle tissue, however, there is no difference between these two training exercises.
Full-Text [PDF 539 kb]   (1269 Downloads)    
• For people who suffer from obesity and for counteracting the destructive effects of obesity on fat oxidation factors, including desnutrin, continuous training can be an effective method.
• For weight loss, however, high-intensity interval training is the best method.

Type of Study: Original Article | Subject: Sport Physiology and its related branches
Received: 2017/09/12 | Accepted: 2017/11/27

1. Eckel RH, Kahn SE, Ferrannini E, Goldfine AB, Nathan DM, Schwartz MW, et al. Obesity and type 2 diabetes: what can be unified and what needs to be individualized? Diabetes Care. 2011;34(6):1424-30. [DOI:10.2337/dc11-0447] [PMID] [PMCID]
2. Knapp M, Gorski J. The skeletal and heart muscle triacylglycerol lipolysis revisited. J Physiol Pharmacol. 2017;68(1):3-11. [PMID]
3. Wolf G. The mechanism and regulation of fat mobilization from adipose tissue: desnutrin, a newly discovered lipolytic enzyme. Nutr Rev. 2005;63(5):166-70. [DOI:10.1111/j.1753-4887.2005.tb00134.x] [PMID]
4. Watt MJ, Cheng Y. Triglyceride metabolism in exercising muscle. Biochim Biophys Acta. 2017. [DOI:10.1016/j.bbalip.2017.06.015] [PMID]
5. Villena JA, Roy S, Sarkadi-Nagy E, Kim KH, Sul HS. Desnutrin, an adipocyte gene encoding a novel patatin domain-containing protein, is induced by fasting and glucocorticoids: ectopic expression of desnutrin increases triglyceride hydrolysis. J Biol Chem. 2004;279(45):47066-75. [DOI:10.1074/jbc.M403855200] [PMID]
6. Ahmadian M, Abbott MJ, Tang T, Hudak CS, Kim Y, Bruss M, et al. Desnutrin/ATGL is regulated by AMPK and is required for a brown adipose phenotype. Cell Metab. 2011;13(6):739-48. [DOI:10.1016/j.cmet.2011.05.002] [PMID] [PMCID]
7. Larsen S, Danielsen JH, Sondergard SD, Sogaard D, Vigelsoe A, Dybboe R, et al. The effect of high-intensity training on mitochondrial fat oxidation in skeletal muscle and subcutaneous adipose tissue. Scand J Med Sci Sports. 2015;25(1):e59-69. [DOI:10.1111/sms.12252] [PMID]
8. Morton TL, Galior K, McGrath C, Wu X, Uzer G, Uzer GB, et al. Exercise Increases and Browns Muscle Lipid in High-Fat Diet-Fed Mice. Front Endocrinol (Lausanne). 2016;7:80. [DOI:10.3389/fendo.2016.00080]
9. Ahmadian M, Duncan RE, Varady KA, Frasson D, Hellerstein MK, Birkenfeld AL, et al. Adipose overexpression of desnutrin promotes fatty acid use and attenuates diet-induced obesity. Diabetes. 2009;58(4):855-66. [DOI:10.2337/db08-1644] [PMID] [PMCID]
10. Hoshino D, Yoshida Y, Kitaoka Y, Hatta H, Bonen A. High-intensity interval training increases intrinsic rates of mitochondrial fatty acid oxidation in rat red and white skeletal muscle. Appl Physiol Nutr Metab. 2013;38(3):326-33. [DOI:10.1139/apnm-2012-0257] [PMID]
11. Zhang H, Tong TK, Qiu W, Zhang X, Zhou S, Liu Y, et al. Comparable Effects of High-Intensity Interval Training and Prolonged Continuous Exercise Training on Abdominal Visceral Fat Reduction in Obese Young Women. J Diabetes Res. 2017;2017:5071740. [DOI:10.1155/2017/5071740] [PMID] [PMCID]
12. Alsted TJ, Nybo L, Schweiger M, Fledelius C, Jacobsen P, Zimmermann R, et al. Adipose triglyceride lipase in human skeletal muscle is upregulated by exercise training. Am J Physiol Endocrinol Metab. 2009;296(3):E445-53. [DOI:10.1152/ajpendo.90912.2008] [PMID]
13. Afzalpour ME, Chadorneshin HT, Foadoddini M, Eivari HA. Comparing interval and continuous exercise training regimens on neurotrophic factors in rat brain. Physiol Behav. 2015;147:78-83. [DOI:10.1016/j.physbeh.2015.04.012] [PMID]
14. Storlien LH, James DE, Burleigh KM, Chisholm DJ, Kraegen EW. Fat feeding causes widespread in vivo insulin resistance, decreased energy expenditure, and obesity in rats. Am J Physiol. 1986;251(5 Pt 1):E576-83. [DOI:10.1152/ajpendo.1986.251.5.E576]
15. Ogasawara J, Sakurai T, Kizaki T, Ishibashi Y, Izawa T, Sumitani Y, et al. Higher levels of ATGL are associated with exercise-induced enhancement of lipolysis in rat epididymal adipocytes. PLoS One. 2012;7(7):e40876. [DOI:10.1371/journal.pone.0040876] [PMID] [PMCID]
16. Yao-Borengasser A, Varma V, Coker RH, Ranganathan G, Phanavanh B, Rasouli N, et al. Adipose triglyceride lipase expression in human adipose tissue and muscle. Role in insulin resistance and response to training and pioglitazone. Metabolism. 2011;60(7):1012-20. [DOI:10.1016/j.metabol.2010.10.005] [PMID] [PMCID]
17. Bae JY, Woo J, Roh HT, Lee YH, Ko K, Kang S, et al. The effects of detraining and training on adipose tissue lipid droplet in obese mice after chronic high-fat diet. Lipids Health Dis. 2017;16(1):13. [DOI:10.1186/s12944-016-0398-x] [PMID] [PMCID]
18. Louche K, Badin PM, Montastier E, Laurens C, Bourlier V, de Glisezinski I, et al. Endurance exercise training up-regulates lipolytic proteins and reduces triglyceride content in skeletal muscle of obese subjects. J Clin Endocrinol Metab. 2013;98(12):4863-71. [DOI:10.1210/jc.2013-2058] [PMID]
19. Kim SJ, Tang T, Abbott M, Viscarra JA, Wang Y, Sul HS. AMPK Phosphorylates Desnutrin/ATGL and Hormone-Sensitive Lipase To Regulate Lipolysis and Fatty Acid Oxidation within Adipose Tissue. Mol Cell Biol. 2016;36(14):1961-76. [DOI:10.1128/MCB.00244-16] [PMID] [PMCID]
20. Koichiro Azuma YO, Shogo Tabata, Fuminori Katsukawa, Hiroyuki Ishida, Yuko Oguma, Toshihide Kawai, Hiroshi Itoh, Shigeo Okuda, Shuji Oguchi, Atsumi Ohta, Haruhito Kikuchi, Mitsuru Murata, Hideo Matsumoto. Decrease in regional body fat after long-term high-intensity interval training. The Journal of Physical Fitness and Sports Medicine 2017;16:103-10. [DOI:10.7600/jpfsm.6.103]

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Annals of Applied Sport Science

Designed & Developed by : Yektaweb