year 6, Issue 2 (Summer 2018)                   Ann Appl Sport Sci 2018, 6(2): 45-53 | Back to browse issues page

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Eslami R, Gharakhanlou R, Parnow A. The Response of Skeletal Muscle-Expressed Neurotrophins to Acute Resistance Exercise in Male Wistar Rats. Ann Appl Sport Sci. 2018; 6 (2) :45-53
1- Faculty of Sport Sciences, Allameh Tabataba’i University, Tehran, Iran ,
2- Department of Exercise Physiology, Faculty of Humanity, Tarbiat Modares University, Tehran, Iran
3- Faculty of Sport Sciences, Razi-e-Kermanshah University, Kermanshah, Iran
Abstract:   (3102 Views)
Background. BDNF and NT-4/5 have been proposed to be involved in the coordinated adaptations of the neuromuscular system to the elevated level of activity, but an activity-dependent expression of neurotrophins in skeletal muscle is not well established.
Objectives. We, therefore, investigated the effect of one session of resistance exercise on mRNA expression of some neurotrophins in Slow and fast muscles of Wistar rats.
Methods. The resistance training protocol consisted of climbing a 1-meter–long ladder, with a weight attached to a tail sleeve. Twenty-four hours following the main training session, Soleus and Flexor Hallucis Longus (FHL) muscles were removed. mRNA expression of BDNF, NT4/5, TrkB and p75 proteins was assessed by Quantitative RT-PCR.
Results. The data analysis showed that one session of resistance
exercise significantly (p<0.05) decreased mRNA expression of NT4/5 in soleus muscle, but not in FHL muscle. No significant effects of one resistance exercise bout were detected for BDNF and trkB. Our results also show that p75 mRNA levels in the soleus muscle were significantly elevated (7folds) after one resistance training bout (p<0.05).
Conclusion. The results indicate differential control of BDNF and NT-4/5 expression following resistance exercise in skeletal muscle. Also, we have provided evidence supporting the role of the p75 receptor in neurotrophins response to resistance exercise as a mechanical stimulus.
Full-Text [PDF 623 kb]   (793 Downloads)    

• It seems that neurotrophins and their receptors are main factors in muscle response to resistance exercise as a mechanical stimulus.
• Increased activity as resistance training has a potential 
for skeletal muscle remodeling via alteration in neurotrophins expression.

Type of Study: Original Article | Subject: Sport Physiology and its related branches
Received: 2018/02/20 | Accepted: 2018/04/29 | Published: 2018/08/18 | ePublished: 2018/08/18

1. Beaumont E, Gardiner PF. Endurance training alters the biophysical properties of hindlimb motoneurons in rats. Muscle Nerve 2003; 27: 228–236. [DOI:10.1002/mus.10308] [PMID]
2. Gomez-Pinilla F, Ying Z, Opazo P, Roy RR, Edgerton VR. Differential regulation by exercise of BDNF and NT-3 in rat spinal cord and skeletal muscle. Eur J Neurosci 2001;13:1078–1084. [DOI:10.1046/j.0953-816x.2001.01484.x] [PMID]
3. Chevrel G, Hohlfeld R, Sendtner R. The role of neurotrophins in muscle under physiological and pathological conditions. Muscle Nerve 2006; 33: 462–476. [DOI:10.1002/mus.20444] [PMID]
4. Munson JB, Foehring RC, Mendell LM, Gordon T. Fast-to-slow conversion following chronic low-frequency activation of medial gastrocnemius muscle in cats II. Motoneuron properties. J Neurophysiol 1997;77:2605–2615. [DOI:10.1152/jn.1997.77.5.2605] [PMID]
5. Beaumont E, Gardiner PF. Endurance training alters the biophysical properties of hindlimb motoneurons in rats. Muscle Nerve 2003;27: 228–236. [DOI:10.1002/mus.10308] [PMID]
6. Ogborn DI, Gardiner PF. Effects of exercise and muscle type on BDNF, NT-4/5, and TrkB expression in skeletal muscle. Muscle Nerve 2010; 41: 385–391. [DOI:10.1002/mus.21503] [PMID]
7. Gomez-Pinilla F, Ying Z, Roy RR, Molteni R, Edgerton VR. Voluntary exercise induces a BDNF-mediated mechanism that promotes neuroplasticity. J Neurophysiol 2002; 88: 2187–2195. [DOI:10.1152/jn.00152.2002] [PMID]
8. Thomas SM, DeMarco M, D'Arcangelo G, Halegoua S, Brugge JS. Ras is essential for nerve growth factor- and phorbol ester-induced tyrosine phosphorylation of MAP kinases. Cell. 1992; 68(6):1031-40. [DOI:10.1016/0092-8674(92)90075-N]
9. Hennigan A, O'Callaghan RM, Kelly AM. Neurotrophins and their receptors: rolesin plasticity, neurodegeneration an neuroprotection. Biochemical Society Transactions 2007; 35: 424-427. [DOI:10.1042/BST0350424] [PMID]
10. Cuppini R, Sartini S, Agostini D, Guescini M, Ambrogini P, Betti M, et al. BDNF expression in rat skeletal muscle after acute or repeated exercise. Arch Ital Biol 2007;145:99–110. [PMID]
11. Klein R, Lamballe F, Bryant S, Barbacid M. The trkB tyrosine protein kinase is a receptor for neurotrophin-4. Neuron 1992;8:947–956. [DOI:10.1016/0896-6273(92)90209-V]
12. Klein R, Nanduri V, Jing SA, Lamballe F, Tapley P, Bryant S, et al. The trkB tyrosine protein kinase is a receptor for brain-derived neurotrophic factor and neurotrophin-3. Cell 1991;66:395–403. [DOI:10.1016/0092-8674(91)90628-C]
13. Godfrey JK, Kayser BD, Gomez GV, Bennett J, Jaque SV, Sumida KD. Interrupted resistance training and BMD in growing rats. Sports Med 2009; 30: 579 – 584. [DOI:10.1055/s-0029-1202823]
14. Lee S and Farrar RP. Resistance training induces muscle-specific changes in muscle mass and function in rat. JEP online 2003; 6(2):80-87.
15. Forsgren S, Bergh A, Carlsson E, and Thornell LE. Calcitonin gene-related peptide expression at endplates of different fibre types in muscles in rat hind limbs. Cell and Tissue Research 1993; 274(3): 439–446. [DOI:10.1007/BF00314540] [PMID]
16. Fontaine B, Klarsfeld A, Hokfeld T, and Changeux JP. Calcitonin gene-related peptide, a peptide present in spinal cord motoneurons, increases the number of acetylcholine receptors in primary cultures of chick embryo myotubes. Neuroscience Letters 1986; 71: 59–65. [DOI:10.1016/0304-3940(86)90257-0]
17. Kenneth JL and Thomas DS. Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2-ΔΔCT Method. Methods 2001; 25: 402–408. [DOI:10.1006/meth.2001.1262] [PMID]
18. Sakuma K, and Yamaguchi A. The Recent Understanding of the Neurotrophin's Role in SkeletalMuscle Adaptation. Journal of Biomedicine and Biotechnology 2011; doi:10.1155/2011/201696. [DOI:10.1155/2011/201696]
19. Griesbeck O, Parsadanian AS, Sendtner M, and Thoenen H. Expression of neurotrophins in skeletal muscle: quantitative comparison and significance for motoneuron survival and maintenance of function. Journal of Neuroscience Research 1995; 42: 21–33. [DOI:10.1002/jnr.490420104] [PMID]
20. Ip FCF, Cheung J, and Ip NY. The expression profiles of neurotrophins and their receptors in rat and chicken tissues during development," Neuroscience Letters 2001; 301 (2): 107–110. [DOI:10.1016/S0304-3940(01)01603-2]
21. Koliatsos VE, Cayouette MH, Berkemeier LR, Clatterbuck R E, Price DL, and Rosenthal A. Neurotrophin4/5 is a trophic factor for mammalian facial motor neurons. Proceedings of the National Academy of Sciences of the United States of America 1994; 91 (8): 3304–3308. [DOI:10.1073/pnas.91.8.3304] [PMID]
22. Koliatsos VE, Clatterbuck RE, Winslow JW, Cayouette MH, and Price DL. Evidence that brain-derived neurotrophic factor is a trophic factor for motor neurons invivo. Neuron 1993; 10 (3): 359–367. [DOI:10.1016/0896-6273(93)90326-M]
23. Maisonpierre PC, Belluscio L, Friedman B et al. NT-3, BDNF, and NGF in the developing rat nervous system: parallel as well as reciprocal patterns of expression," Neuron 1990; 5(4): 501–509. [DOI:10.1016/0896-6273(90)90089-X]
24. Sakuma K, Watanabe K, Sano M, Uramoto I, Nakano H, Li YJ, et al. A possible role for BDNF, NT-4 and TrkB in the spinal cord and muscle of rat subjected to mechanical overload, bupivacaine injection and axotomy. Brain Res 2001; 907: 1–19. [DOI:10.1016/S0006-8993(01)02288-0]
25. Schecterson LC and Bothwell M. Novel roles for neurotrophins are suggested by BDNF and NT-3 mRNA expression in developing neurons," Neuron 1992; 9(3): 449–463. [DOI:10.1016/0896-6273(92)90183-E]
26. Oppenheim RW, Qin-Wei Y, Prevette D, and Yan Q. Brain-derived neurotrophic factor rescues developing avian motoneurons fromcell death. Nature1992; 360(6406): 755–757. [DOI:10.1038/360755a0] [PMID]
27. Zhang XH and Poo MM. Localized synaptic potentiation by BDNF requires local protein synthesis in the developing axon. Neuron 2002; 36 (4): 675–688. [DOI:10.1016/S0896-6273(02)01023-1]
28. Funakoshi H, Belluardo N, Arenas E, Yamamoto Y, Casabona A, Persson H and Ibanez CF. Musclederived neurotrophin-4 as an activity-dependent trophic signal for adult motor neurons. Science 1995; 268: 1495–1499. [DOI:10.1126/science.7770776] [PMID]
29. Mousavi K, Parry DJ, and Jasmin B J. BDNF rescues myosin heavy chain IIB muscle fibers after neonatal nerve injury. American Journal of Physiology 2004; 287: C22–C29. [DOI:10.1152/ajpcell.00583.2003] [PMID]
30. Funakoshi H, Frisen J, Barbany G, Timmusk T, Zachrisson O, Verge VM, et al. Differential expression of mRNAs for neurotrophins and their receptors after axotomy of the sciatic nerve. J Cell Biol 1993;123:455–465. [DOI:10.1083/jcb.123.2.455] [PMID]
31. Deschenes M R, Wilson MH. Age-Related Differences in Synaptic Plasticityfollowing Muscle Unloading. J Neurobiol 2003; 57: 246–256. [DOI:10.1002/neu.10271] [PMID]
32. Carrasco DI, and English AW. Neurotrophin 4/5 is required for the normal development of the slow muscle fiber phenotype in the rat soleus. J Exp Biol 2003; 206: 2191-2200. [DOI:10.1242/jeb.00412]
33. Fry AC. The role of resistance exercise intensity on muscle fibre adaptations. Sports Med 2004; 34 (10): 663-679. [DOI:10.2165/00007256-200434100-00004] [PMID]
34. Wang XH, and Poo MM. Potentiation of developing synapses by postsynaptic release of neurotrophin-4. Neuron 1997; 19: 825–835. [DOI:10.1016/S0896-6273(00)80964-2]
35. Wells DG, McKechnie BA, Kelkar S, Fallon JR. Neurotrophins regulate agrin-induced postsynaptic differentiation. Proc. Natl. Acad. Sci, 1999; 96: 1112–1117. [DOI:10.1073/pnas.96.3.1112] [PMID]
36. Walker UA, Schon EA. Neurotrophin-4 is up-regulated in ragged-red fibers associated with pathogenic mitochondrial DNA mutations. Ann Neurol 1998; 43: 536–540. [DOI:10.1002/ana.410430421] [PMID]
37. Mantilla CB, Sieck GC. Trophic factor expression in phrenic motor neurons. Respiratory Physiology & Neurobiology 2008; 164:252–262. [DOI:10.1016/j.resp.2008.07.018] [PMID] [PMCID]
38. Simon M, Porter R, Brown R, Coulton GR, Terenghi G. Effect of NT-4 and BDNF delivery to damaged sciatic nerves on phenotypic recovery of fast and slow muscles fibres. Eur. J. Neurosci 2003; 18: 2460–2466. [DOI:10.1046/j.1460-9568.2003.02978.x] [PMID]
39. Barbacid M. The Trk family of neurotrophin receptors. J Neurobiol 1994; 25: 1386 –1403. [DOI:10.1002/neu.480251107] [PMID]
40. Bibel M, Hoppe E, Barde YA. Biochemical and functional interactions between the neurotrophin receptors trk and p75NTR. EMBO J 1999;18: 616–622. [DOI:10.1093/emboj/18.3.616] [PMID] [PMCID]
41. Mousavi K, Jasmin BJ. BDNF is expressed in skeletal muscle satellite cells and inhibits myogenic differentiation. J Neurosci 2006; 26: 5739-5749. [DOI:10.1523/JNEUROSCI.5398-05.2006] [PMID]
42. Deponti D, Buono R, Catanzaro G, Palma CD, Longhi R, Meneveri R, et al. The Low-Affinity Receptor for Neurotrophins p75NTR Plays a Key Role for Satellite Cell Function in Muscle Repair Acting via RhoA. Molecular Biology of the Cell 2009; 20: 3620–3627. [DOI:10.1091/mbc.e09-01-0012] [PMID] [PMCID]
43. Colombo E, Romaggi S, Blasevich F, Mora M, Falcone C, Lochmüller H, et al. The neurotrophin receptor p75NTR is induced on mature myofibres in inflammatory myopathies and promotes myotube survival to inflammatory stress. Neuropathology and Applied Neurobiology 2012; 38(4): 367–378. [DOI:10.1111/j.1365-2990.2011.01212.x] [PMID]

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