year 6, Issue 1 (Spring 2018)                   Ann Appl Sport Sci 2018, 6(1): 21-28 | Back to browse issues page

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Hosseinzadeh M, Rashid Lamir A, Hejazi S M. Are Heart Muscle Irisin Levels and FNDC5 Gene Expression Regulated by Endurance and Resistance Exercises?. Ann Appl Sport Sci 2018; 6 (1) :21-28
1- Department of Physical Education, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran
2- Department of Exercise Physiology, Faculty of Sport Science, Ferdowsi University of Mashhad, Mashhad, Iran ,
3- Department of Physical Education, Mashhad Branch, Islamic Azad University, Mashhad, Iran
Abstract:   (7732 Views)
Background. Irisin is the result of the proteolysis of fibronectin type III domain-containing protein 5, the levels of which are determined by physical activity.
Objective(s). This study aims to identify changes in FNDC5 gene expression and the heart muscle irisin levels in male rats following 8 weeks resistance and endurance training
Methods. Fifteen male rats (10-12 weeks old and 331.8±63.09 g in weight) were randomly assigned to three groups: control, resistance
and endurance training groups (5 in each group). The endurance group performed activities on a treadmill for 8 weeks. The resistance group performed activities for 8 weeks on a one-meter-long ladder with a slope of 85○. The control group did not undergo any particular training. Sampling was carried out 72 h after the end of the training. The heart tissue were excised, cleaned and immediately frozen in liquid nitrogen, and stored at -70○C. FNDC5 gene expression and the heart muscle irisin levels were measured using Real-Time PCR and ELISA, respectively.
Results. Kruskal-Wallis test showed a significant increase in the heart muscle FNDC5 expression in both training groups (P=0.004). One-way ANOVA showed a significant difference in heart muscle irisin levels among the groups (p=0.004).
Conclusion. It can be concluded that both resistance and endurance training can significantly increase FNDC5 gene expression and the heart muscle irisin levels, suggesting that physical activity can prevent and cure cardiovascular diseases.
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• Irisin is a peptide which is synthesized in the heart muscle and can directly inhibit the formation of atheroma by stimulating the conversion of white adipose tissue into brown adipose tissue, thus increasing metabolic uncoupling and hence caloric expenditure in the heart.

Type of Study: Original Article | Subject: Sport Physiology and its related branches
Received: 2017/05/13 | Accepted: 2017/08/5

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