year 8, Issue 2 (Summer 2020)
Ann Appl Sport Sci 2020, 8(2): 0-0 |
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Heiat F, Ghanbarzadeh M, Ranjbar R, Shojaeifard M. Continuous Swimming Training Arises a Remarkable Effect on Some Longevity Biomarkers in Rat Skeletal Muscles. Ann Appl Sport Sci 2020; 8 (2)
URL: http://aassjournal.com/article-1-769-en.html
URL: http://aassjournal.com/article-1-769-en.html
1- Department of Exercise Physiology, Faculty of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2- Department of Exercise Physiology, Faculty of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran ,ghanbarzadeh313@gmail.com
3- Department of Physiology, Fasa University of Medical Sciences, Fasa, Iran
2- Department of Exercise Physiology, Faculty of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran ,
3- Department of Physiology, Fasa University of Medical Sciences, Fasa, Iran
Abstract: (4197 Views)
Background. SIRT3 is one of the members of the Sirtuin deacetylase protein family which is linked to the longevity of human being and is used as an important therapeutic and diagnostic marker in illness and aging.
Objectives. The aim of this study was studying the effect of continuous swimming training on some biomarkers of longevity in slowtwitch (SOL) and fast-twitch (EDL) muscles of adult male rats. Methods. 20 healthy male, 5 months old rats were divided into two groups: control (n = 10) and exercise (n = 10) groups. Continuous swimming training was performed 5 days a week for 8 weeks, including 30 minutes of workout without adding weights in the first week to 60 minutes of workout, adding weights by 3% of the body weight in the eighth week. Twenty four hours after the last exercise session, SOL and EDL muscles were removed and the changes in variables (PGC-1α, SIRT3, and GSH: GSSG) were measured by ELISA method.
Results. The results showed that conducting 8 weeks of continuous swimming training significantly increased PGC-1α and SIRT3 levels in slow-twitch (SOL) and fast-twitch (EDL) muscles (p<0.05). There was no significant difference in the aging index (GSH: GSSG) of the SOL and EDL groups compared to the control group (p>0.05).
Conclusion. According to the findings of the present study, the implementation of continuous swimming exercises can improve the PGC-1α and SIRT3 proteins, which are biogenesis mitochondrial and life span biomarkers in slow- and fast-twitch muscles.
Objectives. The aim of this study was studying the effect of continuous swimming training on some biomarkers of longevity in slowtwitch (SOL) and fast-twitch (EDL) muscles of adult male rats. Methods. 20 healthy male, 5 months old rats were divided into two groups: control (n = 10) and exercise (n = 10) groups. Continuous swimming training was performed 5 days a week for 8 weeks, including 30 minutes of workout without adding weights in the first week to 60 minutes of workout, adding weights by 3% of the body weight in the eighth week. Twenty four hours after the last exercise session, SOL and EDL muscles were removed and the changes in variables (PGC-1α, SIRT3, and GSH: GSSG) were measured by ELISA method.
Results. The results showed that conducting 8 weeks of continuous swimming training significantly increased PGC-1α and SIRT3 levels in slow-twitch (SOL) and fast-twitch (EDL) muscles (p<0.05). There was no significant difference in the aging index (GSH: GSSG) of the SOL and EDL groups compared to the control group (p>0.05).
Conclusion. According to the findings of the present study, the implementation of continuous swimming exercises can improve the PGC-1α and SIRT3 proteins, which are biogenesis mitochondrial and life span biomarkers in slow- and fast-twitch muscles.
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APPLICABLE REMARKS
APPLICABLE REMARKS
According to the results of this study, people are recommended to use continuous exercise to improve mitochondrial biogenesis and delay cell aging.
Type of Study: Original Article |
Subject:
Sport Physiology and its related branches
Received: 2019/06/5 | Accepted: 2019/08/27
Received: 2019/06/5 | Accepted: 2019/08/27
References
1. Robergs RA, Roberts S. Fundamental principles of exercise physiology: for fitness, performance, and health: McGraw-Hill College; 2000.
2. Brand MD, Buckingham JA, Esteves TC, Green K, Lambert AJ, Miwa S, et al., editors. Mitochondrial superoxide and aging: uncoupling-protein activity and superoxide production. Biochemical Society Symposia; 2004: Portland Press Limited. [DOI:10.1042/bss0710203] [PMID]
3. Phaniendra A, Jestadi DB, Periyasamy L. Free radicals: properties, sources, targets, and their implication in various diseases. Indian J Clin Biochem. 2015;30(1):11-26. [DOI:10.1007/s12291-014-0446-0] [PMID] [PMCID]
4. Birben E, Sahiner UM, Sackesen C, Erzurum S, Kalayci O. Oxidative stress and antioxidant defense. World Allergy Organ J. 2012;5(1):9-19. [DOI:10.1097/WOX.0b013e3182439613] [PMID] [PMCID]
5. Pryor WA, Stanley JP, Blair E. Autoxidation of polyunsaturated fatty acids: II. A suggested mechanism for the formation of TBA-reactive materials from prostaglandin-like endoperoxides. Lipids. 1976;11(5):370-9. [DOI:10.1007/BF02532843] [PMID]
6. Taylor EB, Lamb JD, Hurst RW, Chesser DG, Ellingson WJ, Greenwood LJ, et al. Endurance training increases skeletal muscle LKB1 and PGC-1alpha protein abundance: effects of time and intensity. Am J Physiol Endocrinol Metab. 2005;289(6):E960-8. [DOI:10.1152/ajpendo.00237.2005] [PMID]
7. Russell AP, Feilchenfeldt J, Schreiber S, Praz M, Crettenand A, Gobelet C, et al. Endurance training in humans leads to fiber type-specific increases in levels of peroxisome proliferator-activated receptor-gamma coactivator-1 and peroxisome proliferator-activated receptor-alpha in skeletal muscle. Diabetes. 2003;52(12):2874-81. [DOI:10.2337/diabetes.52.12.2874] [PMID]
8. Coffey VG, Hawley JA. The molecular bases of training adaptation. Sports Med. 2007;37(9):737-63. [DOI:10.2165/00007256-200737090-00001] [PMID]
9. Rose AJ, Hargreaves M. Exercise increases Ca2+-calmodulin-dependent protein kinase II activity in human skeletal muscle. J Physiol. 2003;553(Pt 1):303-9. [DOI:10.1113/jphysiol.2003.054171] [PMID] [PMCID]
10. Little JP, Safdar A, Wilkin GP, Tarnopolsky MA, Gibala MJ. A practical model of low-volume high-intensity interval training induces mitochondrial biogenesis in human skeletal muscle: potential mechanisms. J Physiol. 2010;588(Pt 6):1011-22. [DOI:10.1113/jphysiol.2009.181743] [PMID] [PMCID]
11. Herzig S, Long F, Jhala US, Hedrick S, Quinn R, Bauer A, et al. CREB regulates hepatic gluconeogenesis through the coactivator PGC-1. Nature. 2001;413(6852):179-83. [DOI:10.1038/35093131] [PMID]
12. Palacios OM, Carmona JJ, Michan S, Chen KY, Manabe Y, Ward JL, 3rd, et al. Diet and exercise signals regulate SIRT3 and activate AMPK and PGC-1alpha in skeletal muscle. Aging (Albany NY). 2009;1(9):771-83. [DOI:10.18632/aging.100075] [PMID] [PMCID]
13. St-Pierre J, Drori S, Uldry M, Silvaggi JM, Rhee J, Jager S, et al. Suppression of reactive oxygen species and neurodegeneration by the PGC-1 transcriptional coactivators. Cell. 2006;127(2):397-408. [DOI:10.1016/j.cell.2006.09.024] [PMID]
14. Steinbacher P, Eckl P. Impact of oxidative stress on exercising skeletal muscle. Biomolecules. 2015;5(2):356-77. [DOI:10.3390/biom5020356] [PMID] [PMCID]
15. Imai S, Armstrong CM, Kaeberlein M, Guarente L. Transcriptional silencing and longevity protein Sir2 is an NAD-dependent histone deacetylase. Nature. 2000;403(6771):795-800. [DOI:10.1038/35001622] [PMID]
16. Sahlin K, Shabalina IG, Mattsson CM, Bakkman L, Fernstrom M, Rozhdestvenskaya Z, et al. Ultraendurance exercise increases the production of reactive oxygen species in isolated mitochondria from human skeletal muscle. J Appl Physiol (1985). 2010;108(4):780-7. [DOI:10.1152/japplphysiol.00966.2009] [PMID] [PMCID]
17. Kincaid B, Bossy-Wetzel E. Forever young: SIRT3 a shield against mitochondrial meltdown, aging, and neurodegeneration. Front Aging Neurosci. 2013;5:48. [DOI:10.3389/fnagi.2013.00048] [PMID] [PMCID]
18. Mockett RJ, Bayne AC, Kwong LK, Orr WC, Sohal RS. Ectopic expression of catalase in Drosophila mitochondria increases stress resistance but not longevity. Free Radic Biol Med. 2003;34(2):207-17. [DOI:10.1016/S0891-5849(02)01190-5]
19. Oh T, Tanaka S, Naka T, Igawa S. Effects of high-intensity swimming training on the bones of ovariectomized rats. J Exerc Nutrition Biochem. 2016;20(3):39-45. [DOI:10.20463/jenb.2016.09.20.3.6] [PMID] [PMCID]
20. da Rocha GL, Crisp AH, de Oliveira MR, da Silva CA, Silva JO, Duarte AC, et al. Effect of High Intensity Interval and Continuous Swimming Training on Body Mass Adiposity Level and Serum Parameters in High-Fat Diet Fed Rats. ScientificWorldJournal. 2016;2016:2194120. [DOI:10.1155/2016/2194120] [PMID] [PMCID]
21. Vieira JM, Carvalho FB, Gutierres JM, Soares MSP, Oliveira PS, Rubin MA, et al. Caffeine prevents high-intensity exercise-induced increase in enzymatic antioxidant and Na(+)-K(+)-ATPase activities and reduction of anxiolytic like-behaviour in rats. Redox report : communications in free radical research. 2017;22(6):493-500. [DOI:10.1080/13510002.2017.1322739] [PMID]
22. Terada S, Tabata I, Higuchi M. Effect of high-intensity intermittent swimming training on fatty acid oxidation enzyme activity in rat skeletal muscle. The Japanese journal of physiology. 2004;54(1):47-52. [DOI:10.2170/jjphysiol.54.47] [PMID]
23. Carnevali LC, Jr., Eder R, Lira FS, Lima WP, Goncalves DC, Zanchi NE, et al. Effects of high-intensity intermittent training on carnitine palmitoyl transferase activity in the gastrocnemius muscle of rats. Braz J Med Biol Res. 2012;45(8):777-83. [DOI:10.1590/S0100-879X2012007500105] [PMID] [PMCID]
24. Long YC, Barnes BR, Mahlapuu M, Steiler TL, Martinsson S, Leng Y, et al. Role of AMP-activated protein kinase in the coordinated expression of genes controlling glucose and lipid metabolism in mouse white skeletal muscle. Diabetologia. 2005;48(11):2354-64. [DOI:10.1007/s00125-005-1962-5] [PMID]
25. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry. 1976;72:248-54. [DOI:10.1016/0003-2697(76)90527-3]
26. Viña J, Borrás C, Miquel J. Theories of ageing. Iubmb Life. 2007;59(4‐5):249-54. [DOI:10.1080/15216540601178067] [PMID]
27. Romano AD, Serviddio G, de Matthaeis A, Bellanti F, Vendemiale G. Oxidative stress and aging. Journal of nephrology. 2010;23 Suppl 15:S29-36.
28. Behnaz F, Soltanpoor P, Teymourian H, Tadayon N, Mohseni GR, Ghasemi M. Sympatholytic and Anti-Inflammatory Effects of Ropivacaine and Bupivacaine After Infraclavicular Block in Arterio Venous Fistula Surgery. Anesth Pain Med. 2019;9(1):e85704. [DOI:10.5812/aapm.85704] [PMID] [PMCID]
29. Wu Z, Puigserver P, Andersson U, Zhang C, Adelmant G, Mootha V, et al. Mechanisms controlling mitochondrial biogenesis and respiration through the thermogenic coactivator PGC-1. Cell. 1999;98(1):115-24. [DOI:10.1016/S0092-8674(00)80611-X]
30. Kamei Y, Ohizumi H, Fujitani Y, Nemoto T, Tanaka T, Takahashi N, et al. PPARgamma coactivator 1beta/ERR ligand 1 is an ERR protein ligand, whose expression induces a high-energy expenditure and antagonizes obesity. Proceedings of the National Academy of Sciences of the United States of America. 2003;100(21):12378-83. [DOI:10.1073/pnas.2135217100] [PMID] [PMCID]
31. Anastasiou D, Krek W. SIRT1: linking adaptive cellular responses to aging-associated changes in organismal physiology. Physiology (Bethesda, Md). 2006;21:404-10. [DOI:10.1152/physiol.00031.2006] [PMID]
32. Kong X, Wang R, Xue Y, Liu X, Zhang H, Chen Y, et al. Sirtuin 3, a new target of PGC-1alpha, plays an important role in the suppression of ROS and mitochondrial biogenesis. PloS one. 2010;5(7):e11707. [DOI:10.1371/journal.pone.0011707] [PMID] [PMCID]
33. Hey-Mogensen M, Goncalves RL, Orr AL, Brand MD. Production of superoxide/H2O2 by dihydroorotate dehydrogenase in rat skeletal muscle mitochondria. Free Radic Biol Med. 2014;72:149-55. [DOI:10.1016/j.freeradbiomed.2014.04.007] [PMID]
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