year 2, Issue 1 (Spring 2014)                   Ann Appl Sport Sci 2014, 2(1): 37-44 | Back to browse issues page

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Ghanbari-Niaki A, Gholizadeh M, Ghanbari-Abarghooi S, Roudbari F, Chaichi M J. Liver ABCA1 Gene Expression in Male Rats: Effects of High-intensity Treadmill Running and Black Crataegus-pentaegyna (Siayh-Valik) Extraction. Ann Appl Sport Sci. 2014; 2 (1) :37-44
1- Exercise Biochemistry Division, Faculty of Physical Education and Sport Science, University of Mazandaran ,
2- Exercise Biochemistry Division, Faculty of Physical Education and Sport Science, University of Mazandaran
3- Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran
4- Analytical Chemistry, Faculty of Chemistry, University of Mazandaran
Abstract:   (13111 Views)

The purpose of the current study was to investigate the effect of a high- intensity treadmill running (8 weeks) with or without aqueous extraction of black Crataegus-Pentaegyna (Siyah -Valik) on liver ABCA1 mRNA expression and plasma HDL-C, total, and direct bilirubin concentration in male rats. Twenty Wistar male rats (4-6 weeks old, 140-170 g weight) were used. Animals were randomly assigned into training (n =10) and control (n =10) groups and further divided into saline-control (SC, n=5), saline-training (ST, n=5), black Crataegus-Pentaegyna (Siyah-Valik) Control (SVC, n=5), and black Crataegus-Pentaegyna (Siyah-Valik)-training (SVT, n=5) groups. Training groups have performed a high-intensity running program (34 m/min on 0% grade, 60 min/day and 5 days/week) on a motor-driven treadmill for 8 weeks. Animals were orally fed with black Crataegus-Pentaegyna (Siyah –Valik) extraction (500 mg/kg body weight) and saline solution for last six weeks. A significant differences have found in liver ABCA1 gene expression between SVC and SVT (P<0.003) and between SVC with SC groups (P< 0.038). HDL-C levels were significantly (P<0.036) between groups. A higher HDL level was found in SV treated groups and between SVC and SC groups. The levels of bilirubin total and bilirubin direct remained unchanged. The current results show that high-intensity treadmill running affected liver ABCA1 mRNA expression in different directions in saline (increase) and SV (decrease) treated animals. Findings also indicate an opposite pattern of change in saline and SV treated animals at rest. It seems the existence of opposite effect of exercise with supplementation of SV might be attributed to the suppression of lignds which is provided by SV supplementation at rest. This in turn might be also taking in account in lower liver ABCA1 mRNA expression and its related nuclear receptors such as LXR in SVT not ST groups.

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Type of Study: Original Article | Subject: Sport Physiology and its related branches
Received: 2013/12/8 | Accepted: 2014/03/30 | Published: 2014/07/6 | ePublished: 2014/07/6

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